Cortexa research tool·genomics·dna_tools

DNA Calculate Gc Content

Calculate GC content percentage and full nucleotide composition of a DNA sequence. Returns GC%, AT%, individual base counts (A, T, G, C, N), and interpretation (low/normal/high GC). Useful for primer design quality checks, cloning efficiency prediction, and sequence characterization. High GC (>60%) sequences may require special PCR conditions.

Livelast probed 2026-04-30 23:07Z· 102msCortexa

Status

The most recent probe succeeded. Safe to call from the agent today.

Last probed: 1d ago
Last success: 1d ago
Last latency: 102ms

Schema

JSON Schema the agent (or your API call) must match.

{
  "properties": {
    "operation": {
      "description": "Operation type",
      "enum": [
        "calculate_gc_content"
      ],
      "type": "string"
    },
    "sequence": {
      "description": "DNA sequence (A, T, G, C, N only). Case insensitive.",
      "type": "string"
    }
  },
  "required": [
    "operation",
    "sequence"
  ],
  "type": "object"
}

Examples (1)

Default DNA Calculate Gc Content call

public-safeinput

{
  "operation": "",
  "sequence": ""
}

Expected response keys: success

Anonymous-safe example. Rate-limited; no sign-in required.

Identifiers

Catalog ID: tu_DNA_calculate_gc_content
Tool name: DNA_calculate_gc_content
Added: 2026-04-30 22:47Z
Tags: tooluniverse

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