DNA Gibson Design

Design Gibson Assembly overlaps for seamless DNA fragment assembly. For each fragment, computes the 5' overhang (from the previous fragment's 3' end) and 3' overhang (from the next fragment's 5' end) based on the specified overlap length (default 20 bp). Returns the modified fragment sequences ready for Gibson Assembly cloning. Use for: seamless cloning, multi-fragment assembly, vector backbone insertion.