Cortexa research tool·structure·dna_tools

DNA Golden Gate Design

Design Golden Gate Assembly parts with Type IIS restriction enzyme sites (BsaI or BbsI). Assigns unique non-palindromic 4-bp overhangs to each junction and builds the full synthetic part sequences including enzyme recognition sites. Use for: scarless multi-part assembly, modular cloning (MoClo), Golden Gate cloning workflows.

Livelast probed 2026-05-01 01:00Z· 85msCortexa

Status

The most recent probe succeeded. Safe to call from the agent today.

Last probed: 1d ago
Last success: 1d ago
Last latency: 85ms

Schema

JSON Schema the agent (or your API call) must match.

{
  "properties": {
    "enzyme": {
      "default": "BsaI",
      "description": "Type IIS restriction enzyme to use. Options: 'BsaI' (default) or 'BbsI'.",
      "enum": [
        "BsaI",
        "BbsI",
        null
      ],
      "type": [
        "string",
        "null"
      ]
    },
    "operation": {
      "description": "Operation type",
      "enum": [
        "golden_gate_design"
      ],
      "type": "string"
    },
    "parts": {
      "description": "List of DNA part sequences to assemble (at least 2). A, T, G, C only.",
      "items": {
        "type": "string"
      },
      "type": "array"
    }
  },
  "required": [
    "operation",
    "parts"
  ],
  "type": "object"
}

Examples (1)

Default DNA Golden Gate Design call

public-safeinput

{
  "operation": "",
  "parts": []
}

Expected response keys: success

Anonymous-safe example. Rate-limited; no sign-in required.

Identifiers

Catalog ID: tu_DNA_golden_gate_design
Tool name: DNA_golden_gate_design
Added: 2026-05-01 00:58Z
Tags: tooluniverse

DNA Golden Gate Design

Livelast probed 2026-05-01 01:00Z· 85msCortexa

Schema

JSON Schema the agent (or your API call) must match.

{ "properties": { "enzyme": { "default": "BsaI", "description": "Type IIS restriction enzyme to use. Options: 'BsaI' (default) or 'BbsI'.", "enum": [ "BsaI", "BbsI", null ], "type": [ "string", "null" ] }, "operation": { "description": "Operation type", "enum": [ "golden_gate_design" ], "type": "string" }, "parts": { "description": "List of DNA part sequences to assemble (at least 2). A, T, G, C only.", "items": { "type": "string" }, "type": "array" } }, "required": [ "operation", "parts" ], "type": "object" }