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Cortexa research tool·genomics·dna_tools
DNA Primer Design
Design forward and reverse PCR primers for a target DNA region using the SantaLucia 1998 nearest-neighbor thermodynamic model. Selects primers with Tm closest to the target (default 60°C), GC content 40-60%, length 18-25 bp, and no 3'-end repeats. Use for: PCR amplification, genotyping, diagnostic assay design, amplicon sequencing.
AvailableCortexa
Schema
JSON Schema the agent (or your API call) must match.
JSON · 58 lines · 1,349 chars
Examples (1)
Default DNA Primer Design call
public-safeinput
JSON · 4 lines · 39 chars
Expected response keys: success
Anonymous-safe example. Rate-limited; no sign-in required.
Identifiers
- Catalog ID
- tu_DNA_primer_design
- Tool name
- DNA_primer_design
- Added
- 2026-05-01 00:58Z
- Tags
- tooluniverse