Cortexa research tool·genomics·dna_tools

DNA Primer Design

Design forward and reverse PCR primers for a target DNA region using the SantaLucia 1998 nearest-neighbor thermodynamic model. Selects primers with Tm closest to the target (default 60°C), GC content 40-60%, length 18-25 bp, and no 3'-end repeats. Use for: PCR amplification, genotyping, diagnostic assay design, amplicon sequencing.

Livelast probed 2026-05-01 01:00Z· 114msCortexa

Status

The most recent probe succeeded. Safe to call from the agent today.

Last probed: 1d ago
Last success: 1d ago
Last latency: 114ms

Schema

JSON Schema the agent (or your API call) must match.

{
  "properties": {
    "operation": {
      "description": "Operation type",
      "enum": [
        "primer_design"
      ],
      "type": "string"
    },
    "product_size_max": {
      "default": 1000,
      "description": "Maximum acceptable PCR product size in bp (default: 1000).",
      "type": [
        "integer",
        "null"
      ]
    },
    "product_size_min": {
      "default": 100,
      "description": "Minimum acceptable PCR product size in bp (default: 100).",
      "type": [
        "integer",
        "null"
      ]
    },
    "sequence": {
      "description": "DNA template sequence (A, T, G, C, N only). Must be at least 200 bp for good primer design.",
      "type": "string"
    },
    "target_end": {
      "description": "0-based end position of the target region to amplify. Defaults to sequence length.",
      "type": [
        "integer",
        "null"
      ]
    },
    "target_start": {
      "description": "0-based start position of the target region to amplify. Defaults to 0.",
      "type": [
        "integer",
        "null"
      ]
    },
    "tm_target": {
      "default": 60,
      "description": "Target melting temperature in Celsius (default: 60.0°C).",
      "type": [
        "number",
        "null"
      ]
    }
  },
  "required": [
    "operation",
    "sequence"
  ],
  "type": "object"
}

Examples (1)

Default DNA Primer Design call

public-safeinput

{
  "operation": "",
  "sequence": ""
}

Expected response keys: success

Anonymous-safe example. Rate-limited; no sign-in required.

Identifiers

Catalog ID: tu_DNA_primer_design
Tool name: DNA_primer_design
Added: 2026-05-01 00:58Z
Tags: tooluniverse

DNA Primer Design

Livelast probed 2026-05-01 01:00Z· 114msCortexa

Schema

JSON Schema the agent (or your API call) must match.

{ "properties": { "operation": { "description": "Operation type", "enum": [ "primer_design" ], "type": "string" }, "product_size_max": { "default": 1000, "description": "Maximum acceptable PCR product size in bp (default: 1000).", "type": [ "integer", "null" ] }, "product_size_min": { "default": 100, "description": "Minimum acceptable PCR product size in bp (default: 100).", "type": [ "integer", "null" ] }, "sequence": { "description": "DNA template sequence (A, T, G, C, N only). Must be at least 200 bp for good primer design.", "type": "string" }, "target_end": { "description": "0-based end position of the target region to amplify. Defaults to sequence length.", "type": [ "integer", "null" ] }, "target_start": { "description": "0-based start position of the target region to amplify. Defaults to 0.", "type": [ "integer", "null" ] }, "tm_target": { "default": 60, "description": "Target melting temperature in Celsius (default: 60.0°C).", "type": [ "number", "null" ] } }, "required": [ "operation", "sequence" ], "type": "object" }