Cortexa research tool·chemistry·dna_tools

DNA Virtual Digest

Perform a virtual restriction enzyme digest on a DNA sequence using the NEB enzyme library (24 enzymes). Returns all resulting fragments with their sequences, lengths, and positions, plus a list of cut sites. Supports both linear and circular DNA. Use for: cloning planning, gel electrophoresis prediction, verifying construct integrity.

Livelast probed 2026-04-30 23:07Z· 86msCortexa

Status

The most recent probe succeeded. Safe to call from the agent today.

Last probed: 1d ago
Last success: 1d ago
Last latency: 86ms

Schema

JSON Schema the agent (or your API call) must match.

{
  "properties": {
    "circular": {
      "default": false,
      "description": "Treat sequence as circular DNA (e.g., plasmid). Default: false (linear).",
      "type": [
        "boolean",
        "null"
      ]
    },
    "enzymes": {
      "description": "List of enzyme names to use for digestion (e.g., ['EcoRI', 'BamHI']). If null, uses all 24 NEB enzymes.",
      "items": {
        "type": "string"
      },
      "type": [
        "array",
        "null"
      ]
    },
    "operation": {
      "description": "Operation type",
      "enum": [
        "virtual_digest"
      ],
      "type": "string"
    },
    "sequence": {
      "description": "DNA sequence (A, T, G, C, N only). Case insensitive.",
      "type": "string"
    }
  },
  "required": [
    "operation",
    "sequence"
  ],
  "type": "object"
}

Examples (1)

Default DNA Virtual Digest call

public-safeinput

{
  "operation": "",
  "sequence": ""
}

Expected response keys: success

Anonymous-safe example. Rate-limited; no sign-in required.

Identifiers

Catalog ID: tu_DNA_virtual_digest
Tool name: DNA_virtual_digest
Added: 2026-04-30 22:47Z
Tags: tooluniverse

DNA Virtual Digest

Livelast probed 2026-04-30 23:07Z· 86msCortexa

Schema

JSON Schema the agent (or your API call) must match.

{ "properties": { "circular": { "default": false, "description": "Treat sequence as circular DNA (e.g., plasmid). Default: false (linear).", "type": [ "boolean", "null" ] }, "enzymes": { "description": "List of enzyme names to use for digestion (e.g., ['EcoRI', 'BamHI']). If null, uses all 24 NEB enzymes.", "items": { "type": "string" }, "type": [ "array", "null" ] }, "operation": { "description": "Operation type", "enum": [ "virtual_digest" ], "type": "string" }, "sequence": { "description": "DNA sequence (A, T, G, C, N only). Case insensitive.", "type": "string" } }, "required": [ "operation", "sequence" ], "type": "object" }