DoseResponse Calculate Ic50

Overview

Determine the half-maximal inhibitory or effective concentration (IC50/EC50) from paired concentration-response data by fitting the Hill sigmoidal equation. Requires a minimum of 4 data points that span the dynamic response range. Output includes EC50 with 95% confidence interval, Hill exponent n, top plateau Emax, bottom plateau Emin, R2 goodness-of-fit, and log10(EC50) for convenient pIC50 conversion. Suited to potency ranking in compound screens, structure-activity relationship analysis, and cross-assay comparisons.

Status

The most recent probe succeeded. Safe to call from the agent today.

Last probed

1d ago

Last success

1d ago

Last latency

4584ms

Schema

JSON Schema the agent (or your API call) must match.

{
  "properties": {
    "concentrations": {
      "description": "Drug concentrations (positive values, e.g., in µM or nM)",
      "items": {
        "type": "number"
      },
      "type": "array"
    },
    "operation": {
      "description": "Operation type",
      "enum": [
        "calculate_ic50"
      ],
      "type": "string"
    },
    "responses": {
      "description": "Percent inhibition or effect values at each concentration",
      "items": {
        "type": "number"
      },
      "type": "array"
    }
  },
  "required": [
    "operation",
    "concentrations",
    "responses"
  ],
  "type": "object"
}

Examples (1)

{
  "concentrations": [],
  "operation": "",
  "responses": []
}

Identifiers

Catalog ID

tu_DoseResponse_calculate_ic50

Tool name

DoseResponse_calculate_ic50

Added

2026-04-30 22:47Z

Tags

tooluniverse