IDT Analyze Oligo
Analyze an oligonucleotide using the IDT OligoAnalyzer API. Calculates melting temperature (Tm), GC content, molecular weight, molar extinction coefficient, and nmol/OD260 and ug/OD260 yields. Tm is calculated using nearest-neighbor thermodynamic parameters (SantaLucia 1998) under user-specified salt conditions (Na+, Mg2+, dNTPs). Supports DNA and RNA oligos up to 200 bases. Use this BEFORE ordering primers/probes to verify Tm, check GC balance, and estimate synthesis yields. Complements NEB_Tm…
Overview
Analyze an oligonucleotide using the IDT OligoAnalyzer API. Calculates melting temperature (Tm), GC content, molecular weight, molar extinction coefficient, and nmol/OD260 and ug/OD260 yields. Tm is calculated using nearest-neighbor thermodynamic parameters (SantaLucia 1998) under user-specified salt conditions (Na+, Mg2+, dNTPs). Supports DNA and RNA oligos up to 200 bases. Use this BEFORE ordering primers/probes to verify Tm, check GC balance, and estimate synthesis yields. Complements NEB_Tm_calculate (which provides polymerase-specific Tm with salt corrections) and DNA_calculate_gc_content (local, no Tm). For self-dimer/homodimer assessment, use IDT_check_self_dimer.
Schema
JSON Schema the agent (or your API call) must match.
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Examples (1)
Default IDT Analyze Oligo call
Public-safe example · rate-limited
Identifiers
- Catalog ID
- tu_IDT_analyze_oligo
- Tool name
- IDT_analyze_oligo
- Added
- 2026-05-01 00:58Z
- Tags
- tooluniverse