IDT Check Self Dimer
Check an oligonucleotide for self-complementarity and homodimer (self-dimer) formation using the IDT OligoAnalyzer API. Returns predicted self-dimer structures with their thermodynamic stability (delta-G in kcal/mol), number of base pairs, and alignment diagrams. A more negative delta-G indicates a more stable (problematic) dimer. Risk assessment: delta-G > -3.0 kcal/mol is low risk, -3.0 to -6.0 is moderate risk, < -6.0 is high risk. Use this tool AFTER designing primers (DNA_primer_design or…
Overview
Check an oligonucleotide for self-complementarity and homodimer (self-dimer) formation using the IDT OligoAnalyzer API. Returns predicted self-dimer structures with their thermodynamic stability (delta-G in kcal/mol), number of base pairs, and alignment diagrams. A more negative delta-G indicates a more stable (problematic) dimer. Risk assessment: delta-G > -3.0 kcal/mol is low risk, -3.0 to -6.0 is moderate risk, < -6.0 is high risk. Use this tool AFTER designing primers (DNA_primer_design or manual) and BEFORE ordering, to verify primers won't form stable homodimers that reduce PCR efficiency. For basic oligo properties (Tm, GC%, MW), use IDT_analyze_oligo instead.
Schema
JSON Schema the agent (or your API call) must match.
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Examples (1)
Default IDT Check Self Dimer call
Public-safe example · rate-limited
Identifiers
- Catalog ID
- tu_IDT_check_self_dimer
- Tool name
- IDT_check_self_dimer
- Added
- 2026-05-01 00:58Z
- Tags
- tooluniverse