NEB Tm Calculate
Calculate primer melting temperature (Tm) and annealing temperature (Ta) using the NEB Tm Calculator API. Uses thermodynamic nearest-neighbor calculations (SantaLucia 1998) with polymerase-specific salt corrections for accurate PCR temperature predictions. Supports all NEB DNA polymerases including Q5, Phusion, Taq, OneTaq, and custom buffer conditions. When two primers are provided, also calculates the recommended annealing temperature (Ta). Complements the local DNA_primer_design tool by prov…
Overview
Calculate primer melting temperature (Tm) and annealing temperature (Ta) using the NEB Tm Calculator API. Uses thermodynamic nearest-neighbor calculations (SantaLucia 1998) with polymerase-specific salt corrections for accurate PCR temperature predictions. Supports all NEB DNA polymerases including Q5, Phusion, Taq, OneTaq, and custom buffer conditions. When two primers are provided, also calculates the recommended annealing temperature (Ta). Complements the local DNA_primer_design tool by providing manufacturer-validated Tm values, though results can diverge by 10+°C between this tool, DNA_primer_design, and IDT_analyze_oligo for the same primer since each uses different nearest-neighbor parameter sets and salt-correction models -- treat cross-tool Tm comparisons as approximate, not interchangeable. Common polymerase codes: 'q5-0' (Q5 High-Fidelity), 'phusion-0' (Phusion HF Buffer), 'taq-0' (Taq Standard Buffer), 'onetaq-0' (OneTaq Standard Buffer). Use NEB_Tm_list_polymerases to see all available product codes.
Schema
JSON Schema the agent (or your API call) must match.
View JSON schemaExpandCollapse
Examples (1)
Default NEB Tm Calculate call
Public-safe example · rate-limited
Identifiers
- Catalog ID
- tu_NEB_Tm_calculate
- Tool name
- NEB_Tm_calculate
- Added
- 2026-05-01 00:58Z
- Tags
- tooluniverse